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Products Fluorescence
Spectrophotometers F-7100 |
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Fluorescence Spectrophotometer
F-7100
Research grade, high sensitivity fluorescence spectrophotometer.
The Hitachi F7100 features the highest sensitivity in
its class and an extremely long lifetime light source
as well as high speed scanning and a host of accessories.
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FL33 |
ETHANOL MEASUREMENT AND
ENZYME ASSAY WITH USE OF AUTOMATIC 4-TURRET ACCESSORY |
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The F-7000 fluorescence spectrophotometer achieves a high
sensitivity (S/N 800:RMS), ultra-high-speed (60,000nm/min)
wavelength scan at the highest level in its class, with
a wide dynamic range up to 6 digits. Furthermore, it is
applied to research, development, and quality control
in a broad range of fields, such as industrial materials,
life sciences, and biotechnology.
The 4-turret accessory used in this experiment automatically
measures, by computer control, the time course of the
fluorescence intensity at regular time intervals through
4 rotating cells installed in a turret. A reaction trace
for an enzyme is used to measure the time course of fluorescence
intensity in plural samples. Previously, the time course
was measured for one sample at a time, so the measurement
time required was proportional to the number of samples.
Application of the automatic 4-turret accessory reduces
measurement time.
An enzyme assay was applied to ethanol measurement in
this experiment. The amount of ethanol in the fish is
increased according to a decline of the freshness, so
this measurement is referred to as the freshness index.
Ethanol is usually measured with a spectrophotometer.
When ethanol is present in trace amounts,
a low concentration measurement is performed with a fluorescence
spectrophotometer. In this experiment, the general enzyme
assay reagent "F-kit ethanol (J.K.International)"
was used. In addition to the enzyme assay, a spectrophotometer
was used to measure ethanol amounts. The analysis conditions
were optimized, and an analytical curve for a standard
solution was created. |
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FL32 |
FLUORESCENCE SPECTRUM MEASUREMENT
METHOD ALLOWING A COMPARISON OF DIFFERENT EQUIPMENT -
SPECTRAL CALIBRATION OF INSTRUMENTAL RESPONSE AND VALIDATION
METHOD, AND MEASUREMENT EXAMPLE FOR A STANDARD FLUORESCENT
MATERIAL |
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Keywords: Fluorescence spectrum, Spectrum calibration,
Instrumental response, Rhodamine B, Quinine sulfate, Standard
material, GLP/GMP program |
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