Lambda Advanced Technology
Products  Fluorescence  F-7100
Fluorescence Spectrophotometer
F-7100

f7100

Research grade, high sensitivity fluorescence spectrophotometer. The Hitachi F7100 features the highest sensitivity in its class and an extremely long lifetime light source as well as high speed scanning and a host of accessories.

  • The highest sensitivity in its class at S/N 1200 or higher (RMS); S/N 360 (Peak to Peak)
  • Long lifetime Xenon light source (2500 hours of use)
  • Compact design
  • 3D time change measurement mode capable of following time changes at multiple wavelengths
  • Expanded measuring time range enabling measurement of long-lasting phosphorescence
  • Corrects for day-to-day variations or instrumental discrepancies in fluorescent intensity
  • Comprehensive file output of three-dimensional measurement data to support fluorescent fingerprint analysis
  • Suitable for applications including industrial materials such as LEDs and solar-cell materials, environmental science, food inspection, and life sciences
   
ITEM DESCRIPTION

Sensitivity (Raman light of water)

Noise: Background S/N 20,000 or above*6 Noise: Peak 1,200 or above*7
Minimum sample volume 0.6 mL (in use of standard 10 mm rectangular cell)*8
Photometric principle Monochromatic light monitoring ratio calculation
Light source 150 W xenon lamp, self-deozonating lamp house
Monochromator Stigmatic concave diffraction grating: 900 lines/mm, F2.2
Brazed wavelength: Excitation side 300 nm, emission side 400 nm
Measuring wavelength range
(on both EX and EM)
200 to 750 nm, and zero-order light
(Expandable up to 900 nm with optional detector)
Bandpass Excitation side: 1, 2.5, 5, 10, 20 nm
Emission side: 1, 2.5, 5, 10, 20 nm
Resolution 1.0 nm (at 546.1 nm)
Wavelength accuracy ±1 nm
Wavelength scan speed 30, 60, 240, 1,200, 2,400, 12,000, 30,000, 60,000 nm/min
Wavelength drive speed 60,000 nm/min
3D measurement time 3 min*9
Response Response from 0 to 98 %:?0.002, 0.004, 0.01, 0.05, 0.1, 0.5, 2, 4 s
Photometric value range -9999 to 9999
Dimensions/weight Spectrophotometer: 620 (W) x 520 (D) x 300 (H) mm (excluding protrusions)/41 kg
Working temperature / humidity 15 to 35°C,45 to 80% (condensation not allowed, 70% or less at 30°C or higher)
Power consumption (spectrophotometer) 100, 115, 220, 230, 240 V AC, 50/60 Hz, 380 VA
FL Solutions program Standard software
Data processing unit PC: Windows®
Printer Printer compatible with Windows®
   
*6 EX 350 nm, Slit 10 nm, Response 4 s
*7 EX 350 nm, Slit 5 nm, Response 2 s
*8 Does not require cell spacer; no slit restriction
*9 EX 200 to 750 nm, Sampling interval 10 nm, EM 200 to 750 nm, Sampling interval 10 nm
FUNCTIONS
  • 3-dimensional measurement
  • Wavelength scan
  • 3-dimensional scan measurement
  • Time scan measurement mode
  • Photometry mode
  • Others
FL33
ETHANOL MEASUREMENT AND ENZYME ASSAY WITH USE OF AUTOMATIC 4-TURRET ACCESSORY

The F-7000 fluorescence spectrophotometer achieves a high sensitivity (S/N 800:RMS), ultra-high-speed (60,000nm/min) wavelength scan at the highest level in its class, with a wide dynamic range up to 6 digits. Furthermore, it is applied to research, development, and quality control in a broad range of fields, such as industrial materials, life sciences, and biotechnology.
The 4-turret accessory used in this experiment automatically measures, by computer control, the time course of the fluorescence intensity at regular time intervals through 4 rotating cells installed in a turret. A reaction trace for an enzyme is used to measure the time course of fluorescence intensity in plural samples. Previously, the time course was measured for one sample at a time, so the measurement time required was proportional to the number of samples. Application of the automatic 4-turret accessory reduces measurement time.
An enzyme assay was applied to ethanol measurement in this experiment. The amount of ethanol in the fish is increased according to a decline of the freshness, so this measurement is referred to as the freshness index. Ethanol is usually measured with a spectrophotometer. When ethanol is present in trace amounts, a low concentration measurement is performed with a fluorescence spectrophotometer. In this experiment, the general enzyme assay reagent "F-kit ethanol (J.K.International)" was used. In addition to the enzyme assay, a spectrophotometer was used to measure ethanol amounts. The analysis conditions were optimized, and an analytical curve for a standard solution was created.

FL32
FLUORESCENCE SPECTRUM MEASUREMENT METHOD ALLOWING A COMPARISON OF DIFFERENT EQUIPMENT - SPECTRAL CALIBRATION OF INSTRUMENTAL RESPONSE AND VALIDATION METHOD, AND MEASUREMENT EXAMPLE FOR A STANDARD FLUORESCENT MATERIAL

Keywords: Fluorescence spectrum, Spectrum calibration, Instrumental response, Rhodamine B, Quinine sulfate, Standard material, GLP/GMP program